putative ketoacyl-CoA synthase
LOCUS AI736487 537 bp mRNA EST 14-JUN-1999
DEFINITION sb29c09.y1 Gm-c1009 Glycine max cDNA clone GENOME SYSTEMS CLONE ID:
Gm-c1009-233 5' similar to TR:O48780 O48780 PUTATIVE
BETA-KETOACYL-COA SYNTHASE. ;, mRNA sequence.
ACCESSION AI736487
NID g5058011
VERSION AI736487.1 GI:5058011
KEYWORDS EST.
SOURCE soybean.
ORGANISM Glycine max
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicots; Rosidae; eurosids I; Fabales; Fabaceae; Papilionoideae;
Glycine.
REFERENCE 1 (bases 1 to 537)
AUTHORS Shoemaker,R., Briner,D., Marra,M., Hillier,L., Kucaba,T.,
Martin,J., Beck,C., Wylie,T., Underwood,K., Steptoe,M.,
Theising,B., Allen,M., Bowers,Y., Person,B., Swaller,T.,
Gibbons,M., Pape,D., Harvey,N., Schurk,R., Ritter,E., Kohn,S.,
Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R. and
Wilson,R.
TITLE Public Soybean EST Project
JOURNAL Unpublished (1999)
COMMENT
Contact: Shoemaker R/Public Soybean EST Project
Public Soybean EST Project
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available through: Genome Systems, Inc. 4633 World
Parkway Circle St. Louis, Missouri 63134 For further information
call: (800) 430-0030 or (314) 427-3222 FAX:(888) 919-3324 or (314)
427-3324 or contact: clones@genomesystems.com or
info@genomesystems.com web site: www.genomesystems.com
Possible reversed clone: similarity on wrong strand
Seq primer: -40RP from Gibco
High quality sequence stop: 426.
FEATURES Location/Qualifiers
source 1..537
/organism="Glycine max"
/note="Vector: pBluescript II XR; Site_1: EcoRI; Site_2:
XhoI; The mRNA was isolated from entire roots of
2-month-old 'Williams' plants that were greenhouse grown
in 5-gallon pots. To suppress nodulation, Black Gold All-
Purpose potting soil was supplemented with: 0.36g/L
available phosphoric acid (P2O5), 20mg/L urea N, 0.16g/L
S, 0.49mg/L B, 2.5mg/L Cu, 0.15g/L Fe, 13.53mg/L Mn,
0.26mg/L Mo, 14mg/L Zn, 20mg/L Ca, and the following
nutrients in a slow-release form (Osmocote): 0.165g/L
ammonia N, 0.185g/L nitrate N, 0.35g/L available
phosphoric acid, and 0.35g/L soluble potash. No nodules
were visible on the roots at harvest. Stratagene's cDNA
Synthesis Kit (catalog #200401) was used to synthesize
the cDNA. First-strand synthesis was performed with
5-methyl dCTP, hence the ligated cDNA is hemimethylated.
Stratagene's first-strand synthesis primer was used
[GAGAGAGAGAGAGAGAGAGAACTAGTCTCGAG(T)-18]. After
second-strand synthesis, the cDNA ends were 'polished'
with clone Pfu DNA polymerase, ligated to EcoRI adapters,
and phosphorylated. The XhoI site within the first-strand
synthesis primer was restricted by digestion with XhoI;
all XhoI sites in the cDNA would be protected by their
hemimethylated status. The cDNA constructs were
size-fractionated with a 400bp cutoff, using a SizeSep 400
Spun column from Pharmacia. The column eluent was then
ligated into Stratagene's pBluescript II XR Predigested
vector (pBluescript II SK(+) that had been digested with
EcoRI and XhoI, and phosphorylated). Both the white and
blue colonies appear to contain recombinant plasmids with
cDNA inserts."
/db_xref="taxon:3847"
/clone="GENOME SYSTEMS CLONE ID: Gm-c1009-233"
/clone_lib="Gm-c1009"
/lab_host="XL10-Gold"
BASE COUNT 150 a 111 c 122 g 152 t 2 others
ORIGIN
1 aaccctactc catcactttc ggcaatgatt gtcaatcatt acaagctgcg gggaaatata
61 aagagctaca accttggtgg gatgggttgc agtgcagggc tgatctcaat tgatcttgct
121 aaagatcttc tccaagccaa tcccaactcc tatgcattgg tcattagcat ggagaatatc
181 acgttgaatt ggtattttgg aaacgatcgg tcaaagcttg tctctaattg tttggttcgc
241 atgggaggag ctgcagttct gctctccaat aaaagctcgg atagaagaag atccaaatac
301 cgattggtca ccacggttcg cactcataag ggtgctgatg acaagtgctt tagctgtgta
361 acccaagaag aagatgccaa tggcaaggtt ggtgttactt tgtcaaaaga tctgatggct
421 gttgctggtg atgctttgaa aaccaatatc actacactgn ggcctctcgt acttccaaca
481 tctgaacaac tgctattctt ttgcacgtta gttgcaaana agattttcaa gatgaga
//
Score E
Sequences producing significant alignments: (bits) Value
gb|AAB95298.1| (AC003105) putative beta-ketoacyl-CoA syntha... 312 2e-84
gi|1045614 (U37088) beta-ketoacyl-CoA synthase [Simmondsia ... 287 3e-77
gb|AAC34858.1| (AF082033) senescence-associated protein 15 ... 285 1e-76
gi|3142289 (AC002411) Strong similarity to beta-keto-Coa sy... 280 4e-75
gi|4091810 (AF053345) fatty acid elongase 3-ketoacyl-CoA sy... 275 2e-73
gb|AAD22309.1|AC007047_18 (AC007047) putative beta-ketoacyl... 273 5e-73
emb|CAA18830.1| (AL023094) putative ketoacyl-CoA synthase [... 260 5e-69
gi|881615 (U29142) fatty acid elongase 1 [Arabidopsis thali... 248 3e-65
gi|2271465 (AF009563) 3-ketoacyl-CoA synthase [Brassica napus] 245 1e-64
gi|1255207 (U50771) fatty acid elongase [Brassica napus] 245 2e-64
gb|AAD37122.1|AF129511_1 (AF129511) very-long-chain fatty a... 241 3e-63
gi|3075394 (AC004484) putative beta-ketoacyl-CoA synthase [... 236 1e-61
gi|3831446 (AC005819) putative beta-ketoacyl-CoA synthase [... 236 1e-61
gi|4115364 (AC005957) putative fatty acid elongase [Arabido... 231 4e-60
emb|CAA71898| (Y11007) fatty acid elongation 1 [Brassica ju... 229 1e-59
emb|CAB36702| (AL035521) fatty acid elongase-like protein [... 224 3e-58
gb|AAD27560.1|AF114171_1 (AF114171) putative beta-ketoacyl-... 223 6e-58
emb|CAB41336.1| (AL049711) beta-ketoacyl-CoA synthase like ... 185 2e-46
gb|AAD24372.1|AC007171_8 (AC007171) putative fatty acid elo... 166 1e-40
gb|AAC25109.1| (AF054497) fatty acid elongase 1 [Brassica n... 76 2e-13
gb|AAC25110.1| (AF054498) fatty acid elongase 1 [Brassica n... 76 2e-13
gb|AAC25111.1| (AF054499) fatty acid elongase 1 [Brassica r... 74 7e-13
gb|AAC25112.1| (AF054500) fatty acid elongase 1 [Brassica o... 71 6e-12
sp|P54157|BCSA_BACSU PUTATIVE CHALCONE SYNTHASE (NARINGENIN... 58 6e-08
emb|CAB06631| (Z85982) pks10 [Mycobacterium tuberculosis] 46 2e-04
emb|CAB09101| (Z95617) pks11 [Mycobacterium tuberculosis] 45 3e-04
gb|AAD43969.1|AF121274_1 (AF121274) putative chalcone synth... 44 0.001
gb|AAB48106.1| (U41818) PhlD [Pseudomonas fluorescens] 43 0.002
sp|P48404|CHSB_IPOTR CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 43 0.002
sp|P30080|CHS6_SOYBN CHALCONE SYNTHASE 6 (NARINGENIN-CHALCO... 43 0.002
emb|CAA62682| (X91339) chalcone synthase [Gerbera sp.] 42 0.003
emb|CAA62683| (X91340) chalcone sythase [Gerbera hybrida] 42 0.003
pir||JQ2249 naringenin-chalcone synthase (EC 2.3.1.74) - so... 42 0.004
sp|P48406|CHS5_SOYBN CHALCONE SYNTHASE 5 (NARINGENIN-CHALCO... 42 0.004
sp|P24826|CHS1_SOYBN CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 42 0.004
sp|P19168|CHS3_SOYBN CHALCONE SYNTHASE 3 (NARINGENIN-CHALCO... 42 0.004
sp|P17957|CHS2_SOYBN CHALCONE SYNTHASE 2 (NARINGENIN-CHALCO... 42 0.004
emb|CAA11765.1| (AJ223998) PCZA361.6 [Amycolatopsis orienta... 42 0.004
sp|P48396|CHSB_PHANI CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 41 0.005
sp|P48398|CHSB_IPOPU CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 41 0.005
emb|CAB02650| (Z81011) pks18 [Mycobacterium tuberculosis] 41 0.006
pir||JQ1071 naringenin-chalcone synthase (EC 2.3.1.74) - so... 41 0.008
sp|P23569|CHSY_PUELO CHALCONE SYNTHASE (NARINGENIN-CHALCONE... 41 0.008
sp|P30081|CHS7_SOYBN CHALCONE SYNTHASE 7 (NARINGENIN-CHALCO... 41 0.008
sp|P51069|THS3_ARAHY STILBENE SYNTHASE 3 (RESVERATROL SYNTH... 41 0.008
sp|P20178|THS1_ARAHY STILBENE SYNTHASE 1 (RESVERATROL SYNTH... 41 0.008
pir||S00334 resveratrol synthase (EC 2.3.1.-) (clone pGSG10... 41 0.008
sp|P48391|CHS2_GERHY CHALCONE SYNTHASE 2 (NARINGENIN-CHALCO... 41 0.008
emb|CAA44185| (X62299) resveratrol synthase [Arachis hypogaea] 41 0.008
emb|CAA44184| (X62298) resveratrol synthase [Arachis hypogaea] 41 0.008
dbj|BAA81664.1| (AB009351) chalcone synthase [Citrus sinensis] 41 0.008
sp|P22925|CHSD_PETHY CHALCONE SYNTHASE D (NARINGENIN-CHALCO... 41 0.008
pir||SYPJCD naringenin-chalcone synthase (EC 2.3.1.74) D - ... 41 0.008
dbj|BAA33495| (AB018074) similar to chalcone synthase [Stre... 41 0.008
sp|P51081|CHSA_PEA CHALCONE SYNTHASE 1A (NARINGENIN-CHALCON... 40 0.011
dbj|BAA21788| (AB001819) chalcone synthase [Ipomoea nil] 40 0.011
dbj|BAA21789| (AB001827) chalcone synthase [Ipomoea purpurea] 40 0.011
dbj|BAA78617.1| (AB027606) stilbene synthase [Arachis hypog... 40 0.011
sp|P20077|THS2_ARAHY STILBENE SYNTHASE 2 (RESVERATROL SYNTH... 40 0.011
emb|CAA44186| (X62300) resveratrol synthase [Arachis hypogaea] 40 0.011
gi|1658120 (U74082) chalcone synthase [Ipomoea purpurea] 40 0.011
sp|P48390|CHS1_GERHY CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 40 0.014
gi|2198783 (U84912) chalcone synthase-A [Ipomoea purpurea] 40 0.014
emb|CAA62687| (X91344) chalcone synthase [Onoseris sagittatis] 39 0.019
sp|P16107|CHSY_PETCR CHALCONE SYNTHASE (NARINGENIN-CHALCONE... 39 0.019
dbj|BAA03785| (D16256) chalcone synthase [Daucus carota] 39 0.024
dbj|BAA03784| (D16255) chalcone synthase [Daucus carota] 39 0.024
sp|P51082|CHSB_PEA CHALCONE SYNTHASE 1B (NARINGENIN-CHALCON... 39 0.024
emb|CAA07244| (AJ006779) carrot chalcone synthase 1 [Daucus... 39 0.024
gi|2253115 (AF007097) chalcone synthase [Bromheadia finlays... 39 0.024
gi|2253117 (AF007098) chalcone synthase [Bromheadia finlays... 39 0.024
gi|2253119 (AF007099) chalcone synthase [Bromheadia finlays... 39 0.024
sp|P48400|CHSA_IPOPL CHALCONE SYNTHASE A (NARINGENIN-CHALCO... 39 0.024
dbj|BAA20387| (AB001826) chalcone synthase [Ipomoea purpure... 39 0.024
dbj|BAA21787| (AB001818) chalcone synthase [Ipomoea nil] 39 0.024
emb|CAA62686| (X91343) chalcone synthase [Leibnitzia anandria] 39 0.032
pir||PS0079 naringenin-chalcone synthase (EC 2.3.1.74) H - ... 39 0.032
emb|CAA32736| (X14596) chalcone synthase [Petunia x hybrida] 39 0.032
sp|P48401|CHSA_IPOTF CHALCONE SYNTHASE A (NARINGENIN-CHALCO... 39 0.032
sp|P48403|CHSA_IPOTR CHALCONE SYNTHASE A (NARINGENIN-CHALCO... 39 0.032
sp|P53414|CHS1_SECCE CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 39 0.032
sp|P48397|CHSA_IPOPU CHALCONE SYNTHASE A (NARINGENIN-CHALCO... 39 0.032
gi|1381037 (U15946) chalcone synthase A [Ipomoea purpurea] ... 39 0.032
gi|2198781 (U84911) chalcone synthase-A [Ipomoea purpurea] 39 0.032
gi|2198785 (U84913) chalcone synthase-A [Ipomoea purpurea] 39 0.032
sp|P48402|CHSB_IPOTF CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 38 0.042
gi|2565388 (AF026258) chalcone synthase [Onobrychis viciifo... 38 0.042
emb|CAA07245| (AJ006780) carrot chalcone synthase 2 [Daucus... 38 0.042
gi|2196912 (U84905) chalcone synthase-A [Ipomoea purpurea] ... 38 0.042
gi|2198779 (U84910) chalcone synthase-A [Ipomoea purpurea] 38 0.042
sp|P48385|CHSY_CALCH CHALCONE SYNTHASE (NARINGENIN-CHALCONE... 38 0.042
sp|P51089|CHS_VIGUN CHALCONE SYNTHASE (NARINGENIN-CHALCONE ... 38 0.055
dbj|BAA22045| (D88263) chalcone synthase [Pisum sativum] 38 0.055
dbj|BAA23373| (AB008748) chalcone synthase [Scutellaria bai... 38 0.055
pir||S18136 naringenin-chalcone synthase (EC 2.3.1.74) - ga... 38 0.055
sp|P22928|CHSJ_PETHY CHALCONE SYNTHASE J (NARINGENIN-CHALCO... 38 0.055
sp|Q01287|CHS2_PEA CHALCONE SYNTHASE 2 (NAREGENIN-CHALCONE ... 38 0.055
sp|P49440|CHSY_PHAVU CHALCONE SYNTHASE 17 (NARINGENIN-CHALC... 38 0.055
pir||SYPJCJ naringenin-chalcone synthase (EC 2.3.1.74) J - ... 38 0.055
emb|CAA10511| (AJ131813) chalcone synthase [Catharanthus ro... 38 0.055
gb|AAB95298.1| (AC003105) putative beta-ketoacyl-CoA synthase [Arabidopsis
thaliana]
Length = 509
Score = 312 bits (790), Expect = 2e-84
Identities = 152/179 (84%), Positives = 163/179 (90%)
Frame = +1
Query: 1 NPTPSLSAMIVNHYKLRGNIKSYNLGGMGCSAGLISIDLAKDLLQANPNSYALVISMENI 180
NPTPSLSAM+VNHYKLRGNI SYNLGGMGCSAGLISIDLAK LL + PN+YA+VISMENI
Sbjct: 207 NPTPSLSAMVVNHYKLRGNILSYNLGGMGCSAGLISIDLAKHLLHSIPNTYAMVISMENI 266
Query: 181 TLNWYFGNDRSKLVSNCLVRMGGAAVLLSNKSSDRRRSKYRLVTTVRTHKGADDKCFSCV 360
TLNWYFGNDRSKLVSNCL RMGGAA+LLSNK DRRRSKY LV TVRTHKGADDKCF C+
Sbjct: 267 TLNWYFGNDRSKLVSNCLFRMGGAAILLSNKRWDRRRSKYELVDTVRTHKGADDKCFGCI 326
Query: 361 TQEEDANGKVGVTLSKDLMAVAGDALKTNITTLXPLVLPTSEQLLFFCTLVAXKIFKMR 537
TQEED+ K+GVTLSK+LMAVAGDALKTNITTL PLVLPTSEQLLFF TLV K+FKM+
Sbjct: 327 TQEEDSASKIGVTLSKELMAVAGDALKTNITTLGPLVLPTSEQLLFFATLVGRKLFKMK 385
gi|1045614 (U37088) beta-ketoacyl-CoA synthase [Simmondsia chinensis]
Length = 521
Score = 287 bits (728), Expect = 3e-77
Identities = 140/178 (78%), Positives = 156/178 (86%)
Frame = +1
Query: 1 NPTPSLSAMIVNHYKLRGNIKSYNLGGMGCSAGLISIDLAKDLLQANPNSYALVISMENI 180
NPTPSLS+MIVNHYKLRGNI SYNLGGMGCSAGLISIDLAKDLLQ N+Y LV+S EN+
Sbjct: 216 NPTPSLSSMIVNHYKLRGNILSYNLGGMGCSAGLISIDLAKDLLQVYRNTYVLVVSTENM 275
Query: 181 TLNWYFGNDRSKLVSNCLVRMGGAAVLLSNKSSDRRRSKYRLVTTVRTHKGADDKCFSCV 360
TLNWY+GNDRS L++NCL RMGGAA++LSN+ DRRRSKY+L+ TVRTHKGADDK + CV
Sbjct: 276 TLNWYWGNDRSMLITNCLFRMGGAAIILSNRWRDRRRSKYQLLHTVRTHKGADDKSYRCV 335
Query: 361 TQEEDANGKVGVTLSKDLMAVAGDALKTNITTLXPLVLPTSEQLLFFCTLVAXKIFKM 534
Q+ED N KVGV LSKDLMAVAG+ALK NITTL PLVLP SEQLLFF TLVA K+FKM
Sbjct: 336 LQQEDENNKVGVALSKDLMAVAGEALKANITTLGPLVLPMSEQLLFFATLVARKVFKM 393