putative ketoacyl-CoA synthase
LOCUS AI941411 400 bp mRNA EST 03-AUG-1999
DEFINITION sb89c09.y1 Gm-c1017 Glycine max cDNA clone GENOME SYSTEMS CLONE ID:
Gm-c1017-617 5' similar to TR:O65677 O65677 PUTATIVE KETOACYL-COA
SYNTHASE. ;, mRNA sequence.
ACCESSION AI941411
NID g5688396
VERSION AI941411.1 GI:5688396
KEYWORDS EST.
SOURCE soybean.
ORGANISM Glycine max
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
euphyllophytes; Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicots; Rosidae; eurosids I; Fabales; Fabaceae; Papilionoideae;
Glycine.
REFERENCE 1 (bases 1 to 400)
AUTHORS Shoemaker,R., Briner,D., Marra,M., Hillier,L., Kucaba,T.,
Martin,J., Beck,C., Wylie,T., Underwood,K., Steptoe,M.,
Theising,B., Allen,M., Bowers,Y., Person,B., Swaller,T.,
Gibbons,M., Pape,D., Harvey,N., Schurk,R., Ritter,E., Kohn,S.,
Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R. and
Wilson,R.
TITLE Public Soybean EST Project
JOURNAL Unpublished (1999)
COMMENT
Contact: Shoemaker R/Public Soybean EST Project
Public Soybean EST Project
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available through: Genome Systems, Inc. 4633 World
Parkway Circle St. Louis, Missouri 63134 For further information
call: (800) 430-0030 or (314) 427-3222 FAX:(888) 919-3324 or (314)
427-3324 or contact: clones@genomesystems.com or
info@genomesystems.com web site: www.genomesystems.com
High quality sequence stop: 392.
FEATURES Location/Qualifiers
source 1..400
/organism="Glycine max"
/note="Vector: pBluescript II XR; Site_1: EcoRI; Site_2:
XhoI; This cDNA library was constructed from mRNA isolated
from vegetable buds of field grown plants. The cDNA
library was prepared using the Stratagene pBluescript II
XR library construction kit. Complementary DNA was
synthesized from mRNA using a primer consisting of a poly
(dT) sequence with a XhoI restriction site. EcoRI
adapters were ligated to the blunt-ended cDNA fragments
followed by XhoI digestion. The cDNA fragments were
directionally cloned into the EcoRI-XhoI restriction site
of the pBluescript vector. The ligated cDNA fragments
were transformed into XL10-Gold host cells."
/db_xref="taxon:3847"
/clone="GENOME SYSTEMS CLONE ID: Gm-c1017-617"
/clone_lib="Gm-c1017"
/tissue_type="vegetable buds of field grown plants"
/lab_host="XL10-Gold"
BASE COUNT 97 a 95 c 96 g 112 t
ORIGIN
1 tacaggttgg tgcacgttgt tcgcactcac aaaggcgctg atgacaaagc ctaccgttgc
61 gtttttgaag aggaagacag agaagggaaa gtggggattt cacttcagaa ggatctcatg
121 gctattgcag gtgaggctct caagctaaca ttactactat gggtcctctt gttcttccgg
181 cttccgagca gttgctgttc cttctcactc tcattgggag gaaaatcttc aaccccaaat
241 ggaagcctta tattcctgac ttcaaacaag cctttgagca cttctgcatc cacgctggtg
301 gacgtgctgt gattgatgag ctccagaaga atcttcagct ttctactgaa cacgttgagg
361 cttctagaat gacccttcac aggtttggga acacttcgtc
//
BLASTX 8-16-99: putative ketoacyl-CoA synthase
Score E
Sequences producing significant alignments: (bits) Value
gb|AAD37122.1|AF129511_1 (AF129511) very-long-chain fatty a... 168 6e-59
gb|AAD22309.1|AC007047_18 (AC007047) putative beta-ketoacyl... 148 3e-52
gb|AAB95298.1| (AC003105) putative beta-ketoacyl-CoA syntha... 139 3e-47
gi|4091810 (AF053345) fatty acid elongase 3-ketoacyl-CoA sy... 136 6e-47
gi|1045614 (U37088) beta-ketoacyl-CoA synthase [Simmondsia ... 125 4e-46
emb|CAA18830.1| (AL023094) putative ketoacyl-CoA synthase [... 132 4e-46
gi|3142289 (AC002411) Strong similarity to beta-keto-Coa sy... 135 8e-46
gb|AAC34858.1| (AF082033) senescence-associated protein 15 ... 116 7e-43
emb|CAB36702| (AL035521) fatty acid elongase-like protein [... 123 2e-40
gi|4115364 (AC005957) putative fatty acid elongase [Arabido... 128 3e-40
gi|881615 (U29142) fatty acid elongase 1 [Arabidopsis thali... 114 8e-39
gi|3831446 (AC005819) putative beta-ketoacyl-CoA synthase [... 113 2e-37
gi|3075394 (AC004484) putative beta-ketoacyl-CoA synthase [... 108 4e-37
gb|AAD27560.1|AF114171_1 (AF114171) putative beta-ketoacyl-... 94 4e-36
gb|AAC25112.1| (AF054500) fatty acid elongase 1 [Brassica o... 113 1e-29
gb|AAC25109.1| (AF054497) fatty acid elongase 1 [Brassica n... 117 3e-26
gi|2271465 (AF009563) 3-ketoacyl-CoA synthase [Brassica napus] 117 3e-26
gb|AAC25110.1| (AF054498) fatty acid elongase 1 [Brassica n... 116 1e-25
gb|AAC25111.1| (AF054499) fatty acid elongase 1 [Brassica r... 115 1e-25
gi|1255207 (U50771) fatty acid elongase [Brassica napus] 114 3e-25
emb|CAA71898| (Y11007) fatty acid elongation 1 [Brassica ju... 101 4e-21
gb|AAD24372.1|AC007171_8 (AC007171) putative fatty acid elo... 74 1e-19
emb|CAB41336.1| (AL049711) beta-ketoacyl-CoA synthase like ... 55 1e-16
emb|CAA62682| (X91339) chalcone synthase [Gerbera sp.] 41 0.005
emb|CAA62683| (X91340) chalcone sythase [Gerbera hybrida] 40 0.009
sp|P48391|CHS2_GERHY CHALCONE SYNTHASE 2 (NARINGENIN-CHALCO... 39 0.012
gi|3420729 (AF076333) chalcone synthase A1 [Brassica napus] 39 0.021
sp|P30078|CHSY_MALDO CHALCONE SYNTHASE (NAREGENIN-CHALCONE ... 38 0.035
sp|P20178|THS1_ARAHY STILBENE SYNTHASE 1 (RESVERATROL SYNTH... 38 0.035
pir||S00334 resveratrol synthase (EC 2.3.1.-) (clone pGSG10... 38 0.035
dbj|BAA78617.1| (AB027606) stilbene synthase [Arachis hypog... 38 0.035
sp|P51069|THS3_ARAHY STILBENE SYNTHASE 3 (RESVERATROL SYNTH... 38 0.035
emb|CAA44185| (X62299) resveratrol synthase [Arachis hypogaea] 38 0.035
dbj|BAA29039| (AB015430) chalcone synthase-like protein [Hu... 38 0.035
gi|1167950 (U43494) chalcone synthase-like protein [Hordeum... 38 0.046
gi|3420735 (AF076336) chalcone synthase B2 [Brassica napus] 38 0.046
sp|P13417|CHS3_SINAL CHALCONE SYNTHASE 3 (NARINGENIN-CHALCO... 38 0.046
sp|P13416|CHS1_SINAL CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 38 0.046
prf||1609233A chalcone synthase 3 [Sinapis alba] 38 0.046
gi|3420731 (AF076334) chalcone synthase A2 [Brassica napus] 38 0.046
gi|3420733 (AF076335) chalcone synthase B1 [Brassica napus] 38 0.046
gi|2642606 (AF031922) chalcone synthase [Raphanus sativus] 38 0.046
emb|CAA32496| (X14315) chalcone synthase [Sinapis alba] >gi... 37 0.061
dbj|BAA21788| (AB001819) chalcone synthase [Ipomoea nil] 37 0.061
dbj|BAA21789| (AB001827) chalcone synthase [Ipomoea purpurea] 37 0.061
sp|P48401|CHSA_IPOTF CHALCONE SYNTHASE A (NARINGENIN-CHALCO... 37 0.061
sp|P49440|CHSY_PHAVU CHALCONE SYNTHASE 17 (NARINGENIN-CHALC... 37 0.079
sp|P48398|CHSB_IPOPU CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 37 0.079
sp|P48408|DPS2_PINST PINOSYLVIN SYNTHASE 2 (STILBENE SYNTHA... 37 0.079
emb|CAA05214| (AJ002156) chalcone synthase-like protein [Pi... 37 0.079
pir||JQ2249 naringenin-chalcone synthase (EC 2.3.1.74) - so... 37 0.079
sp|P48406|CHS5_SOYBN CHALCONE SYNTHASE 5 (NARINGENIN-CHALCO... 37 0.079
sp|P30080|CHS6_SOYBN CHALCONE SYNTHASE 6 (NARINGENIN-CHALCO... 37 0.079
sp|P24826|CHS1_SOYBN CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 37 0.079
sp|P19168|CHS3_SOYBN CHALCONE SYNTHASE 3 (NARINGENIN-CHALCO... 37 0.079
emb|CAB45446.1| (AL079347) chalcone synthase-like protein [... 37 0.079
sp|P51083|CHS1_TRISU CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 36 0.10
sp|P51084|CHS2_TRISU CHALCONE SYNTHASE 2 (NARINGENIN-CHALCO... 36 0.10
sp|P51085|CHS3_TRISU CHALCONE SYNTHASE 3 (NARINGENIN-CHALCO... 36 0.10
dbj|BAA22042| (D88260) chalcone synthase [Pisum sativum] 36 0.10
dbj|BAA22043| (D88261) chalcone synthase [Pisum sativum] 36 0.10
sp|P30076|CHS8_MEDSA CHALCONE SYNTHASE 8 (NARINGENIN-CHALCO... 36 0.10
dbj|BAA22044| (D88262) chalcone synthase [Pisum sativum] 36 0.10
sp|P51078|CHS5_MEDSA CHALCONE SYNTHASE 4-2 (NARINGENIN-CHAL... 36 0.10
pir||S44368 naringenin-chalcone synthase (EC 2.3.1.74) - al... 36 0.10
sp|P22924|CHSB_PETHY CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 36 0.10
sp|P48394|CHSB_IPOCO CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 36 0.10
sp|P17957|CHS2_SOYBN CHALCONE SYNTHASE 2 (NARINGENIN-CHALCO... 36 0.10
sp|P51080|CHS7_MEDSA CHALCONE SYNTHASE (NARINGENIN-CHALCONE... 36 0.10
sp|P48403|CHSA_IPOTR CHALCONE SYNTHASE A (NARINGENIN-CHALCO... 36 0.10
pir||JQ1071 naringenin-chalcone synthase (EC 2.3.1.74) - so... 36 0.14
gi|166366 (L02903) chalcone synthase [Medicago sativa] 36 0.14
sp|P30073|CHS1_MEDSA CHALCONE SYNTHASE 1 (NARINGENIN-CHALCO... 36 0.14
sp|P30077|CHS9_MEDSA CHALCONE SYNTHASE 9 (NARINGENIN-CHALCO... 36 0.14
sp|P51077|CHS3_MEDSA CHALCONE SYNTHASE 4-1 (NARINGENIN-CHAL... 36 0.14
sp|P30075|CHS4_MEDSA CHALCONE SYNTHASE 4 (NARINGENIN-CHALCO... 36 0.14
sp|P30081|CHS7_SOYBN CHALCONE SYNTHASE 7 (NARINGENIN-CHALCO... 36 0.14
sp|Q01287|CHS2_PEA CHALCONE SYNTHASE 2 (NAREGENIN-CHALCONE ... 36 0.14
sp|Q01288|CHS3_PEA CHALCONE SYNTHASE 3 (NAREGENIN-CHALCONE ... 36 0.14
sp|P51087|CHS5_TRISU CHALCONE SYNTHASE 5 (NARINGENIN-CHALCO... 36 0.14
sp|P51088|CHS6_TRISU CHALCONE SYNTHASE 6 (NARINGENIN-CHALCO... 36 0.14
emb|CAA64452.1| (X94995) naringenin-chalcone synthase [Jugl... 36 0.14
emb|CAA64366.1| (X94706) naringenin-chalcone synthase [Jugl... 36 0.14
dbj|BAA32732| (AB011467) chalcone synthase [Hydrangea macro... 36 0.14
emb|CAA10131| (AJ012690) chalcone synthase [Cicer arietinum] 36 0.14
sp|P30074|CHS2_MEDSA CHALCONE SYNTHASE 2 (NARINGENIN-CHALCO... 36 0.14
emb|CAA10190.1| (AJ012822) chalcone synthase [Cicer arietinum] 36 0.14
pdb|1BI5|A Chain A, Chalcone Synthase From Alfalfa 36 0.14
gi|2317904 (U89959) Similar to rice chalcone synthase homol... 36 0.14
emb|CAA06077| (AJ004800) chalcone synthase [Pinus strobus] 36 0.14
sp|P48396|CHSB_PHANI CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 36 0.14
emb|CAA74847| (Y14507) anther-specific protein [Nicotiana s... 36 0.14
pir||S35165 naringenin-chalcone synthase (EC 2.3.1.74) 4 - ... 36 0.14
sp|P51079|CHS6_MEDSA CHALCONE SYNTHASE 6-4 (NARINGENIN-CHAL... 36 0.14
sp|P51076|CHSY_FRAAN CHALCONE SYNTHASE RJ5 (NARINGENIN-CHAL... 36 0.14
gi|2507617 (U90341) chalcone synthase homolog PrChS1 [Pinus... 36 0.14
sp|P48402|CHSB_IPOTF CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 36 0.18
sp|P48404|CHSB_IPOTR CHALCONE SYNTHASE B (NARINGENIN-CHALCO... 36 0.18
gi|2198779 (U84910) chalcone synthase-A [Ipomoea purpurea] 36 0.18
dbj|BAA81663.1| (AB009350) chalcone synthase [Citrus sinensis] 36 0.18
gb|AAD37122.1|AF129511_1 (AF129511) very-long-chain fatty acid condensing enzyme CUT1
[Arabidopsis thaliana]
Length = 497
Score = 168 bits (420), Expect(2) = 6e-59
Identities = 80/86 (93%), Positives = 83/86 (96%)
Frame = +3
Query: 141 QANITTMGPLVLPASEQLLFLLTLIGRKIFNPKWKPYIPDFKQAFEHFCIHAGGRAVIDE 320
+ANITT+GPLVLPASEQLLFL +LIGRKIFNPKWKPYIPDFK AFEHFCIHAGGRAVIDE
Sbjct: 342 KANITTIGPLVLPASEQLLFLTSLIGRKIFNPKWKPYIPDFKLAFEHFCIHAGGRAVIDE 401
Query: 321 LQKNLQLSTEHVEASRMTLHRFGNTS 398
LQKNLQLS EHVEASRMTLHRFGNTS
Sbjct: 402 LQKNLQLSGEHVEASRMTLHRFGNTS 427
Score = 80.4 bits (195), Expect(2) = 6e-59
Identities = 35/51 (68%), Positives = 45/51 (87%)
Frame = +1
Query: 1 YRLVHVVRTHKGADDKAYRCVFEEEDREGKVGISLQKDLMAIAGEALKLTL 153
Y+L H+VRTH+GADDK++ CV+E+ED+EG VGI+L KDLMAIAGEALK +
Sbjct: 295 YKLSHLVRTHRGADDKSFYCVYEQEDKEGHVGINLSKDLMAIAGEALKANI 345
gb|AAD22309.1|AC007047_18 (AC007047) putative beta-ketoacyl-CoA synthase [Arabidopsis
thaliana]
Length = 512
Score = 148 bits (371), Expect(2) = 3e-52
Identities = 69/86 (80%), Positives = 78/86 (90%)
Frame = +3
Query: 141 QANITTMGPLVLPASEQLLFLLTLIGRKIFNPKWKPYIPDFKQAFEHFCIHAGGRAVIDE 320
+ANITT+GPLVLP SEQ+LF +TL+ +K+FN K KPYIPDFK AF+HFCIHAGGRAVIDE
Sbjct: 361 KANITTLGPLVLPISEQILFFMTLVTKKLFNSKLKPYIPDFKLAFDHFCIHAGGRAVIDE 420
Query: 321 LQKNLQLSTEHVEASRMTLHRFGNTS 398
L+KNLQLS HVEASRMTLHRFGNTS
Sbjct: 421 LEKNLQLSQTHVEASRMTLHRFGNTS 446
Score = 77.3 bits (187), Expect(2) = 3e-52
Identities = 34/51 (66%), Positives = 42/51 (81%)
Frame = +1
Query: 1 YRLVHVVRTHKGADDKAYRCVFEEEDREGKVGISLQKDLMAIAGEALKLTL 153
Y+LVH VRTHKGA +KA+ CV++E+D GK G+SL KDLMAIAGEALK +
Sbjct: 314 YKLVHTVRTHKGAVEKAFNCVYQEQDDNGKTGVSLSKDLMAIAGEALKANI 364