My laboratory studies the molecular mechanisms underlying mammalian spermatogenesis and infertility using molecular, cellular, biochemical, mouse genetics and genomics approaches. The lab is broadly interested in genes and pathways influencing germ/stem cell self-renewal, differentiation and fertility with a current focus on the function of Tudor domain proteins and other Piwi-piRNA pathway associated factors in gene silencing, genome stability, epigenetics, stem cell biology and cancer.
Ding D, Liu J, Midic U, Wu Y, Dong K, Melnick A, Latham KE, Chen C. TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun. 2018 Jan 9;9(1):127. doi: 10.1038/s41467-017-02622-w.
hang H, Liu K, Izumi N, Huang H, Ding D, Ni Z, Sidhu SS, Chen C, Tomari Y, Min J. Structural basis for arginine methylation-independent recognition of PIWIL1 by TDRD2. Proc Natl Acad Sci U S A. 2017 Nov 8. pii: 201711486. doi: 10.1073/pnas.1711486114.
Ding D, Liu J, Dong K, Midic U, Hess RA, Xie H, Demireva EY, Chen C. PNLDC1 is essential for piRNA 3’ end trimming and transposon silencing during spermatogenesis in mice. Nat Commun. 2017 Oct 10;8(1):819. doi: 10.1038/s41467-017-00854-4.
Xu C, Bian C, Yang W, Galka M, Ouyang H, Chen C, Qiu W, Liu H, Jones AE, Mackenzie F, Pan P, Li SS, Wang H, Min J. Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2). Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19266-7.
Liu K*, Chen C*, Guo Y*, Lam R, Bian C, Xu C, Zhao DY, Jin J, MacKenzie F, Pawson T, Min J. Structural basis for recognition of arginine methylated Piwi proteins by the extended Tudor domain. Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18398-403. *Equal contribution.
Chen C, Jin J, James DA, Adams-Cioaba MA, Park JG, Guo Y, Tenaglia E, Xu C, Gish G, Min J, Pawson T. Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20336-41.
Jin J, Xie X*, Chen C*, Park JG, Stark C, James DA, Olhovsky M, Linding R, Mao Y, Pawson T. Eukaryotic protein domains as functional units of cellular evolution. Science Signaling. 2009 Nov 24;2(98):ra76. *Equal contribution.
Chen C, Ouyang W, Grigura V, Zhou Q, Lim H, Zhao GQ, Carnes K, Arber S, Kurpios N, Murphy TL, Cheng AM, Hassell JA, Chandrashekar V, Hofmann M, Hess RA, Murphy KM. ERM is required for transcriptional control of the spermatogonial stem cell niche. Nature. 2005 August 18; 436:1030-34.
ANS815 - Advanced Topics in Reproduction and Development (Course director) ANS425 - Animal Biotechnology ANS409 - Advancements in Reproduction