Fisheries and Wildlife GSO Seminar Series

October 17, 2019 3:30PM - 4:30PM

338, Natural Resources Building


Contact: Courtney Harrison harr1579@msu.edu or Rachel Menale menalera@msu.edu

“Functional studies and vaccine development of
Infectious Pancreatic Necrosis virus and Novirhabdoviruses
affecting Salmonid species”

 

Since commercial production of seafood employs high-density rearing methods, it tends to expose large numbers of aquatic species to infectious diseases. Some of the most important viral pathogens affecting salmonid are Infectious Pancreatic Necrosis virus (IPNV) and Novirhabdoviruses, such as Infectious Hematopoietic Necrosis virus (IHNV), and Viral Hemorrhagic Septicemia virus (VHSV). An outbreak of such viral infections can destroy an entire aquaculture operation. To study the function of viral proteins involved in virulence and pathogenesis, we developed reverse genetics systems for IPNV, IHNV and VHSV. Using this methodology, we identified the amino acid residues involved in virulence and cell culture adaptation of IPNV. Similarly, for VHSV, we recently demonstrated that the nucleoprotein and phosphoprotein are major determinants of the VHSV virulence in rainbow trout. In an effort to develop a subunit vaccine for IPNV, we expressed the structural protein genes in a Baculovirus expression system. Expression of these genes in insect cells resulted in the synthesis of capsid proteins that self-assembled to form "virus-like" particles of IPNV. A single dose of the injectable recombinant subunit IPNV vaccine evoked a protective response against IPNV challenge in Atlantic salmon post-smolts and exhibited significantly lower cumulative mortality rate (35%) than the control fish (77%). For Novirhabdoviruses, we explored the potential of using IHNV as a viral vector and expressed the host-protective hemagglutinin protein of Infectious Salmon Anemia virus (ISAV) on the surface of the virion. Using this approach, it is feasible to develop an attenuated virus-vectored vaccine that gives dual protection against both IHNV and ISAV.

Thursday, October 17, 2019
Room 338 Natural Resources building, 3:30 pm 

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