College of Agriculture & Natural Resources Department of Plant, Soil and Microbial Sciences

• Mariam Sticklen's publications on Google Scholar.

Overview of current program:

Prof. Sticklen research is on production of industrial enzymes and polymers in crop vegetative residues via genetic engineering. Her team also develops drought and salt tolerant crops via the use of transcription factors.

Courses taught: 

• CSS 222: New Horizons in Biotechnology
• CSS 491: Biotechnology: Demystifying the concept

Grants Awarded:   

• Numerous - Department of Energy and USDA/CPBR

Publications:

1. Mariam Sticklen (2013). Inaugural Editorial: Co-Production of High-Value Recombinant Biobased Matter in Bioenergy Crops for Expediting the Cellulosic Biofuels Agenda. Advances in Crop Sci. and Technology. 1(1): 1-2.
2. Thang Nguyen, Hussien Alameldin, Wayne Loescher and Mariam Sticklen (2013). Transgene Pyramiding of the HVA1 and mtlD in T3 Maize (Zea mays L.) Plants Confers Drought and/or Salt Tolerance, along with an Increase in Crop Biomass. Intl. J. Agronomy. Accepted upon revisions.
3. Thang Nguyen and Mariam Sticklen (2013). Barley HVA1 Gene Confers Drought and Salt Tolerance in Transgenic Maize (Zea Mays L.). Advances in Crop Sci. & Technology. In ln press
4. Thang Nguyen, Hussien Alameldin, Benjamin Goheen and Mariam Sticklen  (2013). Bacterial Mannitol-1-Phophate Dehydrogenase (mtlD) Transgene Confers Salt Tolerance in the Fourth Generation Transgenic Maize (Zea Mays. L) Plants. Advances in Crop Sci. & Technology. Accepted upon revisions.
5. Jason D Matakas, Venkatesh Balan, William F Carson IV, Dahai Gao, Federica Brandizzi, Steven Kunkel and Mariam SticklenSticklen (2013). Plant-produced recombinant human interleukin-2 and its activity against splenic CD4+T-cells. International Journal of Life Sciences Biotechnology & Pharma Research. 2(2): 193-203.
6. Kingdom Kwapata, Thang Nguyen and Mariam Sticklen. (2012). Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes. International Journal of Agronomy. Online: Volume 2012 (2012), Article ID 198960, 8 pages. doi:10.1155/2012/198960.
7. Park S-H, C. Mei, M. Pauly^,, R. Garlock, BE. Dale,  R. Sabzikar, H. Fotoh , T. Nguyen and  M. Sticklen (2012). Down-regulation of Maize Cinnamoyl-CoA Reductase via RNAi Technology Causes Brown Midrib and Improves AFEX^TM-Pretreated Conversion into Fermentable Sugars for Biofuels. Crop Science. 52 (6).
8. Park S-H., C. Ransom., C. Mei, R. Sabzikar , C. Qi, S. Chundawat , B. Dale and M. Sticklen (2011). In the quest of alternatives to microbial cellulase mix production: Corn stover-produced heterologous multi-cellulases readily deconstruct lignocellulosic biomass to fermentable sugars. J Chem Technol Biotechnol
9. Mariam Sticklen (2010). Is large-scale production of biofuels possible? Action BioScience.
10. Mariam Sticklen (2009). Expediting biofuels agenda via genetic manipulations of BioEnergy crops. Biofuels, Bioproducts & Biorefining. Biofpr. 3(4): 448-455.
11. Mariam Sticklen (2009). The biofuel agenda shall not wait for miracles: an innovative science and good policy to make a global change in the history of human kind. Biofuels, Bioproducts & Biorefining. Biofpr. 3:419–421.
12. Mariam Sticklen (2007). Feedstock genetic engineering for biofules. Crop Science. 47: 2238-2248.
13. Hesham Oraby,  Balan Venkatesh, Bruce Dale, Rashid Ahmad, Callista Ransom, James Oehmke and Mariam Sticklen (2007). Enhanced conversion of plant biomass into glucose using transgenic rice-produced endoglucanase for cellulosic ethanol. Transgenic Research. 16(6): 739-749.
14. Mariam Sticklen (2006). Plant genetic engineering to improve biomass characterization for biofuels. Current Opinion in Biotechnology. 17(3): 315-319.
15. Chuansheng Mei, SangHyuck Park, , Robab Sabzikar, Qi, Callista Ransom and Mariam  B. Sticklen. 2008. Green Tissue-Specific Production of a Microbial Endo-Cellulase in Maize (Zea mays L.) Endoplasmic-Reticulum and Mitochondria Converts Cellulose into Fermentable Sugars. Journal of Chemical Technology & Biotechnology (JCTB). Published On Line on Dec 30, 2008.

Issued U.S. Patents:

1. Masomeh B. Sticklen. International Patent # 8,350,123. TEC2008-0013. Transgenic cover plants containing hemicellulase and cellulase which degrade lignin and cellulose to fermentable sugars. Issued: Jan. 08, 2013. (see details)
2. Masomeh B. Ssticklen. International Patent # 8,093,456. TEC2008-0013. Transgenic cover plants containing hemicellulase and cellulase which degrade lignin and cellulose to fermentable sugars. Issued: Jan. 12, 2012.
3. Masomeh B. Sticklen, Bruce Dale and Shahina Maqbool. U.S. Patent #7,696,411 issued: Transgenic plants containing ligninase and cellulose which degrade lignin an cellulose to fermentable sugars, Issued April 13, 2010. (see details)
4. Masomeh B. Sticklen, Bruce E. Dale and Shahina B. Maqbool. U.S. Patent #7,569,745. Continuation-In-Part: Transgenic Plants Containing Ligninase & Cellulase, which degrade Lignin and Cellulose to Fermentable Sugars. August 4, 2009. (licensed to private sector for commercialization).
5. Mariam Sticklen. U.S. Patent Publication #20080235820. Lignin reduction and cellulose increase in crop biomass via genetic engineering. Published on September 25, 2008.
6. Mariam Sticklen. International Patent Pulication #WO/2008/069964. Altering regulation of maize legnin biosynthesis enzymes via RNAi technology. Published on December 6, 2008.
7. Masomeh B. Sticklen, Bruce E. Dale and Shahina B. Maqbool. U.S. Patent #7,371,926. Continuation-In-Part: Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars for biofuel. May 13, 2008. (see details; licensed to private sector for commercialization).
8. Masomeh B. Sticklen. U.S. Patent #20050198704. Chloroplast transgenesis of monocots: Bioconfined genetically engineered monocot crops that will eliminate transgene flow. (see details; Research Contract with private sector).
9. Masomeh B. Sticklen, Bruce E. Dale and Shahina B. Maqbool. U.S. Patent #7,049,485. Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars. Issues, June 1, 2006. (licensed to private sector for commercialization).
10. Masomeh B. Sticklen. U.S. Patent # 6,100,456. Lepidopteran insect resistant transgenic potato plants. Issued August 2000.
11. Heng Zhong and Masomeh B. Sticklen. U.S. Patent Number 5,767,368. Method for producing a cereal plant with foreign DNA. Issued June 16, 1998.
12. Masomeh B. Sticklen. U.S. Patent Number 5,728,382. A supplement to item #3: A chitinase cDNA from a disease resistant American elm tree. Issued March 17, 1998.
13. Masomeh B. Sticklen. U.S. Patent Number 5,539,095. A chitinase cDNA clone from a disease resistant American elm. July 1996.
14. Heng Zhong and Masomeh Sticklen. U.S. Patent Number 5,281,529. Method for in vitro sexual reproduction of corn. Issued January 1994.
15. Heng Zhong, C. Srinivasan and Masomeh B. Sticklen. U.S. Patent Number 5,320,961. Method for in vitro asexual reproduction of corn. Issued June 1994.